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KMID : 0360220200610030267
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Journal of the Korean Ophthalmological Society
2020 Volume.61 No. 3 p.267 ~ p.273
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Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells
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Son Myung-Seo
Baek Min-Ju
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of hydrogen sulfide (H2S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO).
Methods: After exposing primary cultured HTMCs to 0, 50, 100, and 500 μM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 μM NaHS and 10 μM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of H2S and NO.
Results: Greater than 100 μM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 μM NaHS and 10 μM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05).
Conclusions: NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by
NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, H2S at high concentrations may increase trabecular outflow, which may not be associated with NO.
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KEYWORD
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Hydrogen sulfide, Nitric oxide, Permeability, Trabecular meshwork
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